Thesis Ivo van der Bijl
On 23 June 2021 Ivo van der Bijl defended his thesis 'Studies on platelets and wound healing' at the Vrije Universiteit Amsterdam.
Promotores: Prof E Middelkoop MD PhD and Prof JJ van Buul PhD
Copomotores: D de Korte PhD and C Margadant PhD
Venue: Vrije Universiteit, Aula
Summary
This thesis describes several studies on platelets, wound healing and angiogenesis. We found that platelets are functional and contain normal levels of growth factors after burn injury and can be used for autologous PRP therapy. However, there are disadvantages with the use of autologous PRP and therefore we studied the effects of different donor-derived PRP compositions on different wound healing assays. We found that the combination of donor-derived platelets and plasma is effective and superior to platelets and plasma alone. Furthermore, we aimed to identify proteins that regulate angiogenesis, a crucial aspect of wound healing. We uncovered Rab GTPases involved in this process and specific roles for Rab5 isoforms, in particular an unique role for Rab5C in maintenance of VEGFR2 levels. Finally, we describe the reciprocal role of integrins ?5ß1 and ?vß3 in cell migration and angiogenesis.
Chapters
Chapter 1
General introduction
Chapter 2
Activation, function and content of platelets in burn patients abstract
Chapter 3
Allogeneic platelet-rich plasma (PRP) is superior to platelets or plasma alone in stimulating fibroblast proliferation and migration, angiogenesis, and chemotaxis as relevant processes for wound healing abstract
Chapter 4
The endosomal RIN2/Rab5C machinery is required for sprouting angiogenesis through stabilizing VEGFR2 and tip cell formation abstract
Chapter 5
An shRNA screen for endothelial Rab GTPases reveals novel regulators of sprouting angiogenesis
Chapter 6
Reciprocal integrin/integrin antagonism through kindlin-2 and Rho GTPases regulates cell cohesion and collective migration abstract
Chapter 7
Summary and general discussion
Download
Download PhD thesis (unversity repository)