Thesis Rianne Opstelten
On 14 October 2021 Sanquin researcher Rianne Opstelten defended her thesis 'Still waters run deep: Making sense of (regulatory) T cell heterogeneity to improve adoptive cellular therapy' at the University of Amsterdam.
Promotor: Prof RAW van Lier MD PhD
Copromotor: D Amsen PhD
Venue: University of Amsterdam, Agnietenkapel and online
Summary
In this thesis, we explore a subset of proposed thymic-derived Treg cells that can be safely used to generate a potent clinical-grade product for Adoptive Cellular Therapy. We reflect on the presence of this Treg subset in cord blood, which is suggested to be a good source for therapeutic Treg cells. Furthermore, we give a comprehensive step-by-step route of development for human thymic Treg cells, showing how and when this subset originates in the thymus. The surface marker that delineates this Treg population is also present on other T cell and leukocyte subsets, and so we conclude this thesis with an overview of its expression on other cell types and speculate on its function.
Chapters
Chapter 1
General introduction
Chapter 2
Separating the wheat from the chaff: making sense of Treg heterogeneity for better adoptive cellular therapy
Chapter 3
Determining the extent of maternal-foetal chimerism in cord blood Abstract
Chapter 4
GPA33: a marker to identify stable human regulatory T cells Abstract
Chapter 5
Single-cell transcriptomics unravels discrete steps in regulatory T cell development in the human thymus
Chapter 6
GPA33 is expressed on multiple human blood cell types and distinguishes CD4+ central memory T cells with and without effector function Abstract
Chapter 7
General discussion
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Download thesis (university repository)