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Wesley (W) Huisman


Molecular Biology

Research interests

Using multi-virus-specific T cell products isolated from healthy third party blood bank donors to control viruses in immunocompromised patients. For chemotherapy treated patients it takes 3 to 6 months before they have recovered their immune system. In this period CMV, EBV and AdV viruses can be reactivated from their latent stage and cause CMV, EBV or AdV disease, which eventually can result in death. We would like to use virus specific T cells from third party blood bank donors to ensure these patients control the virus reactivation. Several parameters needs to be investigated in order to make this product feasible. What type of cells do you need? How can potent cytolytic T cells be generated? Can we predict possible Graft vs Host Disease (GvHD)? In the end we aim to translate such fundamental insights for the implication of human therapies involving multi-virus specific T cell transplantation.

  • Magnetic-activated cell sorting (MACSĀ©)
  • Fluorescence-activated cell sorting (FACS)
  • Functional assay read-outs (ELISA, intra-IFNy, cytotoxicity assays)
  • Cell culture
2015-present PhD student at the Dept of Hematopoiesis, Sanquin, Amsterdam
2014 Animal Laboratory science; Article 9 certificateā€, LUMC
2013-2015 MSc, Molecular Biology; Animal Biology and Disease Models, University of Leiden
2010-2013 BSc, Biology, University of Leiden
Sanquin publications
Last edited on: 10 December 2015
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