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Prof Timo (TK) van den Berg PhD

Blood Cell Research

Biochemistry and Cell Biology (cum laude), University of Amsterdam, 1988


‘Microenvironments in Lymphoid Tissues’
1992 - Vrije University, Amsterdam

Research interests

My main interest is in macrophages and granulocytes. We are studying fundamental aspects of these cells in the immune system as well as their contributions to disease, including infection, inflammation, atherosclerosis and cancer.

  • Relevant functional in vitro and in vivo assays
  • Cell culture
  • Flow cytometry and cell sorting
  • (Confocal) microscopy
  • (Deep) sequencing
  • (Quantitative) PCR
  • Western blotting
2017-present Professor of  Immunotherapy, especially the role of non-specific immunity in tumor reduction, Vrije Univesiteit, Amsterdam
2010-present Head Dept of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, AMC, Amsterdam
2007-2010 Head Phagocyte Laboratory, Sanquin Research, Landsteiner Laboratory, AMC, Amsterdam
1997-2007 Group leader, Dept of Cell Biology and Immunology, VUMC, Amsterdam 
1994-1997 Postdoctoral fellow, Dept of Cell Biology and Immunology, VUMC, Amsterdam
1993-1994 Postdoctoral fellow, Institute for Molecular Medicine, University of Oxford, UK
1988-1992 PhD training, Dept of Cell Biology and Immunology, VUMC, Amsterdam
Sanquin publications
Other publications

Zwijnenburg PJ, van der Poll T, Florquin S, Polfliet MM, van den Berg TK, Dijkstra CD, Roord JJ, Hack CE, van Furth AM. C1 inhibitor treatment improves host defense in pneumococcal meningitis in rats and mice. J Infect Dis 2007; 196:115-23.

Fabriek BO, Møller HJ, Vloet RP, van Winsen LM, Hanemaaijer R, Teunissen CE, Uitdehaag BM, van den Berg TK, Dijkstra CD. Proteolytic shedding of the macrophage scavenger receptor CD163 in multiple sclerosis. J Neuroimmunol 2007; 187:179-86.

Xu W, Schlagwein N, Roos A, van den Berg TK, Daha MR, van Kooten C. Human peritoneal macrophages show functional characteristics of M-CSF-driven anti-inflammatory type 2 macrophages. Eur J Immunol 2007; 37:1594-9.

Fabriek BO, Polfliet MM, Vloet RP, van der Schors RC, Ligtenberg AJ, Weaver LK, Geest C, Matsuno K, Moestrup SK, Dijkstra CD, van den Berg TK. The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor. Blood 2007; 15:5223-9.

Polfliet MM, Fabriek BO, Daniëls WP, Dijkstra CD, van den Berg TK. The rat macrophage scavenger receptor CD163: expression, regulation and role in inflammatory mediator production. Immunobiology 2006; 211:419-25.

Van den Berg TK, van Beek EM, Buhring HJ, Colonna M, Hamaguchi M, Howard CJ, Kasuga M, Liu Y, Matozaki T, Neel BG, Parkos CA, Sano S, Vignery A, Vivier E, Wright M, Zawatzky R, Barclay AN. A nomenclature for signal regulatory protein family members. J Immunol 2005; 175:7788-9.

Alblas J, Honing H, de Lavalette CR, Brown MH, Dijkstra CD, van den Berg TK. Signal regulatory protein alpha ligation induces macrophage nitric oxide production through JAK/STAT- and phosphatidylinositol 3-kinase/Rac1/NAPDH oxidase/H2O2-dependent pathways. Mol Cell Biol 2005; 25:7181-92.

Van Beek EM, Cochrane F, Barclay AN, van den Berg TK. Signal regulatory proteins in the immune system. J Immunol 2005; 175:7781-7.

Fabriek BO, Dijkstra CD, van den Berg TK. The macrophage scavenger receptor CD163. Immunobiology 2005; 210:153-60. Review.

Van den Berg TK, Kraal G. A function for the macrophage F4/80 molecule in tolerance induction. Trends Immunol 2005; 26:506-9. Review.

Van Winsen LL, Hooper-van Veen T, van Rossum EF, Polman CH, van den Berg TK, Koper JW, Uitdehaag BM. The impact of glucocorticoid receptor gene polymorphisms on glucocorticoid sensitivity is outweighted in patients with multiple sclerosis. J Neuroimmunol 2005; 167:150-6.

Fabriek BO, Van Haastert ES, Galea I, Polfliet MM, Dopp ED, Van Den Heuvel MM, van den Berg TK, De Groot CJ, Van Der Valk P, Dijkstra CD. CD163-positive perivascular macrophages in the human CNS express molecules for antigen recognition and presentation. Glia 2005; 51:297-305.

Van Winsen LM, Muris DF, Polman CH, Dijkstra CD, van den Berg TK, Uitdehaag BM. Sensitivity to glucocorticoids is decreased in relapsing remitting multiple sclerosis. J Clin Endocrinol Metab 2005; 90:734-40.

Madsen M, Moller HJ, Nielsen MJ, Jacobsen C, Graversen JH, van den Berg TK, Moestrup SK Molecular characterization of the haptoglobin.hemoglobin receptor CD163. Ligand binding properties of the scavenger receptor cysteine-rich domain region. J Biol Chem 2004; 279:51561-7.

Van den Berg TK, Honing H, Franke N, van Remoortere A, Schiphorst WE, Liu FT, Deelder AM, Cummings RD, Hokke CH, van Die I. LacdiNAc-glycans constitute a parasite pattern for galectin-3-mediated immune recognition. J Immunol 2004;173:1902-07.

Van den Berg TK, Yoder JA, Litman GW. On the origins of adaptive immunity: innate immune receptors join the tale. Trends in Immunology 2004; 25:11-6.

Honing H, van den Berg TK, Van der Pol SM, Dijkstra CD, Van der Kammen RA,Collard JG, De Vries. RhoA activation promotes transendothelial migration of monocytes via ROCK. J Leukoc Biol 2004; 75:523-82.

Hendriks JJ, de Vries HE, van der Pol SM, van den Berg TK, van Tol EA, Dijkstra CD. Flavonoids inhibit myelin phagocytosis by macrophages; a structure-activity relationship study. Biochem Pharmacol 2003; 65:877-85.

Ledeboer A, Wierinckx A, Bol JG, Floris S, Renardel de Lavalette C, De Vries HE, van den Berg TK, Dijkstra CD, Tilders FJ, van dam AM. Regional and temporal expression patterns of interleukin-10, interleukin-10 receptor and adhesion molecules in the rat spinal cord during chronic relapsing EAE. J Neuroimmunol 2003; 136:94-103.

Van Rijn J, van den Berg J, Teerlink T, Kruyt FA, Schor DS, Renardel de Lavalette AC, van den Berg TK, Jakobs C, Slotman BJ. Changes in the ornithine cycle following ionising radiation cause a cytotoxic conditioning of the culture medium of H35 hepatoma cells. Br J Cancer 2003; 88:447-54.

Zwijnenburg PJ, Polfliet MM, Florquin S, van den Berg TK, Dijkstra CD, van Deventer SJ, Roord JJ, van der Poll T, van Furth AM. CXC-chemokines KC and macrophage inflammatory protein-2 (MIP-2) synergistically induce leukocyte recruitment to the central nervous system in rats. Immunol Lett 2003; 85:1-4.

De Vries HE, Hendriks JJ, Honing H, De Lavalette CR, van der Pol SM, Hooijberg E, Dijkstra CD, van den Berg TK. Signal-regulatory protein alpha-CD47 interactions are required for the transmigration of monocytes across cerebral endothelium. J Immunol 2002; 168:5832-9.

Polfliet MM, van de Veerdonk F, Dopp EA, van Kesteren-Hendrikx EM, van Rooijen N, Dijkstra CD, van den Berg TK. The role of perivascular and meningeal macrophages in experimental allergic encephalomyelitis. J Neuroimmunol 2002; 122:1-8.

Van den Berg TK, Puklavec MJ, Barclay AN, Dijkstra CD. Monoclonal antibodies against rat leukocyte surface antigens. Immunol Rev 2001; 184:109-16.

Van den Berg TK, Dopp EA, Dijkstra CD. Rat macrophages: membrane glycoproteins in differentiation and function. Immunol Rev 2001; 184:45-57.

Polfliet MM, Zwijnenburg PJ, van Furth AM, van der Poll T, Dopp EA, Renardel de Lavalette C, van Kesteren-Hendrikx EM, van Rooijen N, Dijkstra CD, van den Berg TK. Meningeal and perivascular macrophages of the central nervous system play a protective role during bacterial meningitis. J Immunol 2001; 167:4644-50.

Polfliet MM, Goede PH, van Kesteren-Hendrikx EM, van Rooijen N, Dijkstra CD, van den Berg TK. A method for the selective depletion of perivascular and meningeal macrophages in the central nervous system. J Neuroimmunol 2001; 116:188-95.

Van den Berg TK, Nath D, Ziltener H, Fukuda M, Van Die I, Crocker PR. Cutting edge: CD43 functions as a T cell counter-receptor for the macrophage adhesion receptor sialoadhesin (siglec-1). J Immunol 2001; 166:3637-30.

Van den Nieuwenhof IM, Renardel de Lavalette C, Diaz N, Van Die I, van den Berg TK. Differential galactosylation of neuronal and haematopoietic signal regulatory protein- determines its cellular binding specificity. J Cell Science 2001; 114:1321-1329.

Schadee-Eestermans IL, Hoefsmit ECM, van de Ende M, PR Crocker, van den Berg TK. Ultrastructural localisation of sialoadhesin (siglec-1) on macrophages in rodent lymphoid tissues. Immunobiology 2000; 202(4):309-25.

Honing H, van Rooijen N, van den Berg TK. Manipulation of macrophage activities using liposomes. J Liposome Res 2000; 10:359-389.

Van der Goes A, Hoekstra K, van den Berg TK, Dijkstra CD. Dexamethasone promotes phagocytosis and bacterial killing by human monocytes/macrophages in vitro. J Leukocyte Biol 2000; 67:801-7.

Verhage M, Maia AS, Plomp JJ, Brussaard AJ, Heeroma JH, Vermeer H, Toonen RT, Hammer RE, van den Berg TK, Missler M, Geuze HJ, Südhof TC. Synaptic assembly of the brain in the absence of neurotransmitter secretion. Science 2000; 287:864-9.

Khouw IMSL, van Wachem PB, van der Worp RJ, van den Berg TK, de Leij LFMH, van Luyn MJA. Systemic anti-IFN treatment and the role of macrophage subsets in the foreign body reaction to dermal sheep collagen in rats. J Biomed Mat Res 2000; 49:297-304.

Jansen J, Uitdehaag B, Koper JW, van den Berg TK. Glucocorticoid receptor ligand binding in monocytic cells using a microplate assay. Pathobiology 2000; 67:262-4.

Van den Heuvel MM, Tensen CP, van As JH, van den Berg TK, Fluitsma DM, Dijkstra CD, Döpp EA, Doste A, van Gaalen FA, Sorg C, Högger P, Beelen RHJ. Regulation of CD163 on human macrophages: cross-linking of CD163 induces signalling and activation. J Leukocyte Biol 1999; 66:858-66.

Van Die I, Gomord V, Kooyman FNJ, van den Berg TK, Cummings RD, Vervelde L. Core 1,3-fucose is a common modification of N-glacans in parasitic helmonths and constitutes an important epitope for IgE from Haemonchus contortus infected sheep. FEBS Lett 1999; 463:189-93.

Van der Goes A, Brouwer J, Hoekstra K, Roos D, van den Berg TK, Dijkstra CD. Reactive oxygen species are required for the phagocytosis of myelin by macrophages. J Neuroimmunol 1998; 92:67-75.

Hazenbos WLW, Heijnen IAFM, Meyer D, Hofhuis FMA, Delavalette CR, Schmidt RE, Capel PJA, van de Winkel JGJ, Gessner JE, van den Berg TK, Verbeek JS. Murine IgG1 complexes trigger immune effector functions predominantly via Fc-gamma-RIII (CD16). J Immunol 1998; 161:3026-32.

Adams S, van der Laan LJW, Vernon-Wilson E, Renardel de Lavalette C, Döpp EA, Dijkstra CD, Simmons DL, van den Berg TK. Signal-regulatory protein is selectively expressed by myeloid and neuronal cells. J Immunol 1998; 161:1853-9.

Crocker PR, Clark EA, Filbin M, Gordon S, Jones Y, Kehrl JH, Kelm S, Le Douarin N, Powell L, Roder J, Schnaar RL, Sgroi DC, Stamenkovic I, Schauer R, Schachner M, van den Berg TK, van der Merwe A, Watt SM, Varki A. Siglecs: a family of sialic-acid binding lectins. Glycobiology 1998; 8:v-vi.

Veerman ECI, Bolscher JGM, Appelmelk BJ, Bloemena E, van den Berg TK, Nieuw-Amerongen AV. A monoclonal antibody directed against high Mr salivary mucins recognizes the SO3-3Gal(1-3GlcNAc moiety of sulfo-LewisA: a histochemical survey of human and rat tissue. Glycobiology 1997; 7:37-43.

Van Rooijen N, Sanders A, van den Berg TK. Apoptosis of macrophages induced by liposome-mediated intracellular delivery of clodronate and propamidine. J Immunol Methods 1996; 193:93-9.

Van den Berg TK, van DieI, Renardel de Lavalette C, Döpp EA, Smit LD, van der Meide PH, Tilders FJH, Crocker PR, Dijkstra CD. Regulation of sialoadhesin expression on rat macrophages: Induction by glucocorticoids and enhancement by IFN, IFN, IL4, and lipopolysaccharide. J Immunol 1996; 157:3130-8.

Van den Berg TK, Hasbold J, Renardel de Lavalette C, Döpp EA, Dijkstra CD, Klaus GGB. Properties of mouse CD40: differential expression of CD40 epitopes on dendritic cells and epithelial cells. Immunol 1996; 88:294-300.

Kruyt FAE, Dijkmans LM, van den Berg TK, Joenje H. Fanconi anemia genes act to suppress a crosslinker-inducible p53-independent apoptosis pathway in lymphoblastoid cell lines. Blood 1996; 87:38-948.

Yoshida K, Kaji M, Takahashi T, van den Berg TK, Dijkstra CD. Host origin of follicular dendritic cells induced in the spleen of SCID mice after transfer of allogeneic lymphocytes. Immunol 1995; 84:117-26.

Yoshida K, van den Berg TK, Dijkstra CD. The functional state of follicular dendritic cells in severe combined immunodeficient mice: role of lymphocytes. Eur J Immunol 1995; 24: 464-8.

Van den Berg TK, Yoshida K, Dijkstra CD. Mechanism of immune complex trapping by follicular dendritic cells. Curr Top Microbiol Immunol 1995; 201:49-69.

Dijkstra CD, EA Dopp, van den Berg TK, JGMC Damoiseaux. Monoclonal antibodies against rat macrophages. J Immunol Meth 1994; 174:1-23.

Smidt C, Verschueren H, Toussaint-Demylle D, van den Berg T, Kraal G, de Baetselier P. The role of the spleen in the organ-specific metastasis of murine BW5147 T lymphomas. Clin Exp Metastasis 1994; 12:164-74.

Kraal G, Hoeben K,. Breve J, Van den Berg TK. The role of sialic acid in the localization of lymphocytes in the spleen. Immunobiol 1994; 190:138-49.

Barbe E, Dopp EA, Damoiseaux JGMC, van den Berg TK, Dijkstra CD. The role of macrophages in regeneration of splenic tissue after autologous transplantation in rat. Adv Exp Med Biol 1994; 355:57-61.

Van Poppel M, van den Berg TK, van Rees EP, Biewenga J. Reticulum cells in the ontogeny of nasal-associated lymphoid tissue (NALT) in the rat. Cell Tiss Res 1993; 273:577-81.

Yoshida K, van den Berg TK, Dijkstra CD. Two functionally different follicular dendritic cells in secondary lymphoid follicles of mouse spleen, as revealed by CR1/2 and FcRII-mediated immune complex trapping. Immunol 1993; 80:34-9.

Yoshida K, van den Berg TK, Dijkstra CD. Two different mechanisms of immune complex trapping in the mouse spleen during immune responses. Adv Exp Med Biol 1993; 329:377-82.

Van den Berg TK, M. van der Ende, EA Dopp, G. Kraal, Dijkstra CD. Localization of beta1 integrins and their extracellular ligands in human lymphoid tissues. Am J Pathol 1993;143:1098.

Van Oostveen DC, van den Berg TK, JGMC. Damoiseaux, EP van Rees. Macrophage subpopulations and reticulum cells in rat placenta. Cell Tiss Res 1992; 268:513-9.

Soesatyo M, van den Berg TK, van Rees E, Biewenga J, Sminia T. Ontogeny of reticulum cells in the rat intestine and their possible role in the development of the lymphoid microenvironment. Reg Immunol 1992; 4:46-52.

Van den Berg TK, Breve JJP, Damoiseaux JGMC, Dopp EA, Kelm S, Crocker PR, Dijkstra CD, Kraal G. Sialoadhesin on macrophages: its identification as a lymphocyte adhesion molecule. J Exp Med 1992; 176:647-55.

Van den Berg TK, EA Dopp, W.R. Daha, G. Kraal, Dijkstra CD. Selective inhibition of immune complex trapping by follicular dendritic cells with monoclonal antibodies against rat C3. Eur J Immunol 1992; 22:957-62.

Dijkstra CD, van den Berg TK The follicular dendritic cell: possible regulatory role of associated molecules. Res Immunol 1991; 142:227.

Van den Berg TK, Dopp EA, Kraal G, Dijkstra CD. Selective inhibition of immune complex trapping by a monoclonal antibody, ED11, recognizing a follicular dendritic cell-associated molecule. In: Lymphatic tissues and in vivo immune responses. 1991. Eds. B.A. Imhof, S. Berrih-Akhin, S. Ezine.

Scherer E, van den Berg T, Vermeulen E, Winterterp HHK, den Engelse L. Immunocytochemical analysis of O6-alkylguanine shows tissue specific formation and removal from esophageal and liver DNA in rats treated with methylbenzylnitrosamine, dimethylnitrosamine, diethylnitrosamine and ethylnitrosurea. Cancer Lett 1989; 46:21-9.

Van den Berg TK, Dopp EA, Breve JJP, Kraal G, Dijkstra CD. The heterogeneity of the reticulum of rat peripheral lymphoid organs identified by monoclonal antibodies. Eur J Immunol 1989; 19:1747-56.

Last edited on: 6 July 2017
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