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Sietse (SQ) Nagelkerke MD MSc

Blood Cell Research


Research interests

The role of Fc-gamma receptors in the clearance of platelets in platelet transfusion refractoriness. When patients receive a transfusion with platelets, the platelet count does not always increase adequately, a condition that is called platelet transfusion refractoriness. This problem is often caused by allo-antibodies against the transfused platelets. But even in the presence of allo-antibodies, yields of platelet transfusion differ greatly between patients. We hypothesize that these differences could be caused by polymorphisms in Fc-gamma receptors, leukocyte receptors for immunoglobin G. We are genotyping a group of patients with platelet transfusion refractoriness to be able to compare their Fc-gamma receptor profile to a control group. Also, we are setting up in vitro functional assays of phagocytosis with monocyte-derived and spleen macrophages, by which we can have a closer look at the contribution of different Fc-gamma receptors to phagocytosis of IgG-opsonised particles.

  • Flow Cytometry
  • Expression patterns
  • Phagocytosis assays
  • Cell culturing
  • DNA analysis: MLPA
  • qRT-PCR
2010-presentPhD student at Dept Blood Cell research, Sanquin, Amsterdam, The Netherlands
2009-2010Registrar paediatrics at the Academic Medical Center, Amsterdam
2005-2006Research project at Shaw Warren laboratory, Department of Infectious Disease, Massachusetts General Hospital, Boston MA, USA
2001-2008Medicine, Academisch Medisch Centrum
Sanquin publications
Last edited on: 20 September 2013
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