Prof Marieke (SM) van Ham PhD
Medical microbiology and immunology
Genetic and functional studies of Haemophilus influenzae fimbriae
1994 - Max-Planck-Institute for Biology (Tübingen, Germany) and University of Amsterdam
- Research interests
Regulation of BCR-mediated antigen presentation by B cells. Our research focuses on the regulation of BCR-mediated antigen processing and MHC II presentation in antigen-specific B cells and the B-T cell interactions that control the following humoral response. We use Salmonella typhimurium as a model for infectious antigens and have demonstrated that antigen-specific B cells phagocytose bacteria after BCR ligation. We are currently studying the mechanisms of phagocytosis and its consequences for bacterial survival and antibody generation. In addition, we use tetanus toxoid and the therapeutic antibodies adalimumab to investigate the regulation of the antibody response against non-infectious antigens upon frequent antigen reencounter (hyperimmunisation or repeated treatment).
- Biochemical, flow cytometric and confocal analyses
- B-T assays
- Antigen-specific CD4+ T cell priming and recall responses
- Identification of T cell epitopes
- Cytokine assays
- Antibody assays
- siRNA knockdown in primary cells
2010-present Professor of Biological Immunology, Faculty of Science, University of Amsterdam 2005-present Head of the department of Immunopathology, Sanquin Research, Amsterdam 2003-2004 Staff member at the department of Immunopathology, Sanquin Research, Amsterdam 2000-2003 Postdoctoral training at the division of Tumour Biology, Netherlands Cancer Institute, Amsterdam (group. J.J. Neefjes) 1997-2000 Postdoctoral training at the division of Tumour Biology, Netherlands Cancer Institute, Amsterdam (group. J.J. Neefjes) 1994-1997 Scientific staff member at the Human Immunogenetics Laboratory, Imperial Cancer Research Fund, London, United Kingdom 1994 PhD training at the department of Medical Microbiology, Academic Medical Center, University of Amsterdam and the department of Infectious Biology, Max-Planck-Institute for Biology in Tübingen, German
- Sanquin publications
- Other publications
Van Alphen L, van Ham M, Geelen-van den Broek L, and Pieters T. Relationship between secretion of the Anton blood group antigen in saliva and adherence of Haemophilus influenzae to oropharynx epithelial cells. FEMS Microbiol. Immunol 1989; 1:357.
Van Ham SM, Mooi FR, Sindhunata MG, Maris WR, and van Alphen L. Cloning and expression in Escherichia coli of Haemophilus influenzae fimbrial genes establishes adherence to oropharyngeal epithelial cells. EMBO J 1989; 8:3535.
Van Alphen L, Geelen-van den Broek L, and van Ham M. In vivo and in vitro expression of outer membrane components of Haemophilus influenzae. Microb Pathog 1990; 8:279.
Van Alphen L, Geelen-van den Broek L, Blaas L, van Ham M, and Dankert J. Blocking of fimbria-mediated adherence of Haemophilus influenzae by sialyl gangliosides. Infect Immun 1991; 59:4473.
Van Ham SM, van Alphen L, and Mooi FR. Fimbria-mediated adherence and hemagglutination of Haemophilus influenzae. J Infect Dis 1992; 165:S97.
Van Ham SM, van Alphen L, Mooi FR, and. van Putten JP. Phase variation of H. influenzae fimbriae: transcriptional control of two divergent genes through a variable combined promoter region. Cell 1993; 73:1187.
Van Ham SM, van Alphen L, Mooi FR, and van Putten JP. The fimbrial gene cluster of Haemophilus influenzae type b. Mol Microbiol 1994; 13:673.
Van Alphen L, and van Ham SM. Adherence and invasion of Haemophilus influenzae. Rev Med Microbiol 1994; 5:245.
Davies J, Carlstedt I, Nilsson AK, Hakansson A, Sabharwal H, van Alphen L, van Ham M, and Svanborg C. Binding of Haemophilus influenzae to purified mucins from the human respiratory tract. Infect Immun 1995; 63:2485.
Van Ham SM, van Alphen L, Mooi FR, and van Putten JP. Contribution of the major and minor subunits to fimbria-mediated adherence of Haemophilus influenzae to human epithelial cells and erythrocytes. Infect Immun 1995: 63:4883.
Beck S, Belich M, Grüneberg U, Jackson A, Kelly A, Sanseau P, Sanderson F, Trowsdale J, and van Ham M. Organisation and functions of class II genes and molecules. DNA Seq. 1996; 7:21.
Van Ham SM, Grüneberg U, Malcherek G, Bröker I, Melms A, and Trowsdale J. Human histocompatibility leukocyte antigen (HLA)-DM edits peptides presented by HLA-DR according to their ligand binding motifs. J Exp Med.1996; 184:2019.
Grüneberg U, van Ham SM, Malcherek G, Bröker I, Melms A, and Trowsdale J. The structure and function of the novel MHC class II molecule, HLA-DM. Biochem Soc Trans 1997; 25:208S.
Grüneberg U, Rich T, Roucard C, van Ham SM, Charron D, and Trowsdale J. Two widely used anti-DR alpha monoclonal antibodies bind to an intracellular C-terminal epitope. Hum Immunol 1997; 53:34.
Van Ham SM, Tjin EPM, Lillemeier BF, Grüneberg U, van Meijgaarden KE, Pastoors L, Verwoerd D, Tulp A, Canas B, Rahman D, Ottenhoff TH, Pappin DJ, Trowsdale J, and Neefjes J. HLA-DO is a negative modulator of HLA-DM-mediated MHC class II peptide loading. Curr Biol 1997; 7:950.
Ullrich H.J, Döring K, Grüneberg U, Jähnig F, Trowsdale J, and van Ham SM. Interaction between HLA-DM and HLA-DR involves regions that undergo conformational changes at lysosomal pH. Proc Natl Acad Sci U.S.A. 1997; 94:13163.
Geluk F, Eijk PP, van Ham SM, Jansen HM, and van Alphen L. The fimbria gene cluster of nonencapsulated Haemophilus influenzae. Infect Immun 1998; 66:406.
Hitzel C, Grüneberg U, van Ham M, Trowsdale J, and Koch N. Sodium dodecyl sulfate-resistant HLA-DR "superdimer" bands are in some cases class II heterodimers bound to antibody. J Immunol 1999; 162:4671.
Van Ham M, van Lith M, Lillemeier B, Tjin E, Grüneberg U, Rahman D, Pastoors L, van Meijgaarden K, Roucard C, Trowsdale J, Ottenhoff T, Pappin D, and Neefjes. J Modulation of the MHC class II-associated peptide repertoire by HLA-DO. J Exp Med 2000; 191:1127.
Van Ham M, van Lith M, Griekspoor A, and Neefjes J. What to do with HLA-DO? Immunogenetics 2000; 51:765. Review.
Van Lith M, van Ham M, Griekspoor A, Tjin E, Verwoerd D, Calafat J, Janssen H, Reits E, Pastoors L, and Neefjes J. Regulation of MHC class II antigen presentation by sorting of recycling HLA-DM/DO and class II within the multivesicular body. J Immunol 2001; 167:884.
Roucard C, Thomas C, Pasquier M-A, Trowsdale J, Sotto J-J, Neefjes J, and van Ham M. In vivo and in vitro modulation of HLA-DM and HLA-DO is induced by B lymphocyte activation. J Immunol 2001; 167:6849.
Van Lith M, van Ham M, and Neefjes J. Novel polymorphisms in HLA-DOA and HLA-DOB in B cell malignancies. Immunogenetics 2002; 54:591.