Klaske (KAMH) Thiadens
MSc Molecular Biology and Biotechnology
- Research interests
Our research focuses on the selective mRNA translation in erythropoiesis. Of interest are the erythroid specific genes Bag1 (Bcl2-associated anthanogene 1) and Csde1 (cold-shock domain protein e1) that may contribute to impaired mRNA translation in Diamond Blackfan Anaemia (DBA). The RNA-binding protein Csde1 is known to bind the IRES or several transcripts to control IRES-mediated translation, whereas deregulated expression of Bag1 alters translation of transcripts with upstream open reading frames (uORF). My study addresses mainly the mechanisms that control translation of transcripts with uORFs and its biological importance in erythropoiesis.
We are currently implementing a ribosome profiling method with which we can measure the ribosome occupancy on mRNA. Other techniques we use are: Cell culture of erythroblasts, isolation and analysis of polyribosomes to assay translation initiation, protein and mRNA analysis, flow cytometry. In future we also want to implement PAR-CLIP.
2011-present PhD student at Dept of Hematopoiesis, Sanquin Research, Amsterdam 2010 Graduation MSc Molecular Biology and Biotechnology, University of Groningen 2006-2007 Traineeship, Immunology, Netherlands Cancer Institute, Amsterdam 2005 Traineeship, Cancer Genetic Research Group, Otago University, Christchurch, New Zealand 2004 Traineeship, Molecular Genetics, University of Groningen
- Sanquin publications
- Other publications
Peperzak V, Veraar EA, Xiao Y, Babala N, Thiadens K, Brugmans M, Borst J. CD8+ T Cells Produce the Chemokine CXCL10 in Response to CD27/CD70 Costimulation To Promote Generation of the CD8+ Effector T Cell Pool. J Immunol 2013 Aug 12. [Epub ahead of print]