Kim (K) Brussen
BSc Biology, MSc Biomolecular Sciences (Free University Amsterdam) with a focus on molecular and cell biology and immunology. Subject literature thesis: Roundabout receptors, their Slit ligands and the hematopoietic stem cell niche. Postgraduate courses on immunology and cell signalling.
- Research interests
To elucidate the role of the chemorepellent Slit and its receptor Roundabout in hematopoiesis and hematopoietic cell migration. Current experiments are aimed at determining the contribution of Slit and Roundabout to hematopoietic differentiation as well as the molecular mechanisms involved.
- Molecular and cell biology techniques, i.e. cloning and transfection of cells, protein production and purification, isolation and culturing of primary cells, stem and progenitor cell assays (such as CFU-C, CAFC and LTC-CFC).
- Flow cytometry
- Migration assays
- Confocal microscopy
2010-present PhD student at the Dept of Hematopoiesis, Sanquin Research, Amsterdam 2010 MSc degree in Biomolecular Sciences, VU, Amsterdam 2009 Traineeship at the department of Molecular Cell Biology, Sanquin Research, Amsterdam, research subject: the role of the Rac1 C-terminus in regulating CXCR4 surface expression 2008 Traineeship at the department of Medical Microbiology, VU Medical center, Amsterdam, research subject: the ESX-5 secretome of Mycobacterium avium 2007 Traineeship at the department of Molecular Microbiology, Free University AMsterdam, research subject: protein transport in Escherichia coli
- Sanquin publications