Jesse (J) Eernstman
MSc Medical Biology (Radboud University Nijmegen)
- Research interests
The aim of our research is to culture erythrocytes from induced pluripotent stem cells (iPS) with specific blood group genotypes, to reduce donor dependency and prevent allo-immunisation. To establish human iPS we transduce erythroblasts with a polycistronic lentiviral vector expressing Oct4, Sox2, c-Myc, and Klf4. The transduced cells were transferred to a MEF feeder culture and iPS medium. Embryoid bodies can be generated for which the iPS cells were transferred to uncoated plates in medium supplemented with BMP4, VEGF, SCF, TPO, IL-6, IL-3, insulin, and cholesterol rich lipids. After 14 days embryoid bodies generated from erythroblast-derived iPS contained blood islands that were visible because of the presence of red cells. The next challenge is the large scale expansion of iPS-derived erythroblasts and subsequent maturation to hemoglobinised, enucleated red blood cells.
- Flow Cytometry
- Cell Culturing
- Western Blotting
2013-present PhD student at the Dept of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, Amsterdam 2011 ServiceXS, Leiden, Traineeship:
Epigenetics; protocol development methylation analysis using bisulfite conversion. Innovation studies of cytosine methylation and 5-hydroxymethylation
Erasmus MC, Rotterdam, Cell Biology, Diploma/Master thesis: The regulation and involvement of the proteins A disintegrin and metalloproteinase domain 17 (ADAM17) and Interleuking-6 receptor alpha (IL6r) in Cystic Fibrosis (CF) and down-regulation of the epithelial sodium channel (ENaC) in CF primary bronchial epithelial cells by lentiviral shRNA transduction, to monitor basal fluid resorption.
Scope: Cell biological investigation of machinery in Cystic fibrosis-primary bronchial epithelial cells by high-resolution fluorescent microscopy, ELISA and measuring net ion transports
2010-2012 MSc degree in Medical Biology, Radboud University Nijmegen 2009 Occupational Trainee, University of Queensland, Australia
Bachelor thesis: Understanding the effect of ABA (Abscisic acid) on growth of the transformed legume Lotus japonicas.
2005-2009 Bachelor in Biochemistry, HAN University Nijmegen
- Sanquin publications