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Daphne (DC) Thijssen-Timmer PhD


Medical Biology, University of Amsterdam


'Alternative splicing of thyroid hormone receptor alpha transcripts during health and disease'
Promotor: Prof. Dr. W.M. Wiersinga
15 September 2004 - Department of Endocrinology and Metabolism, Academic Medical Centre, University of Amsterdam

Research interests
Megakaryopoiesis: In vitro strategies to manufacture functional platelets from hematopoietic (CD34+) progenitor cells.
Cellular therapies: in particular the GMP production of ATMPs such as mesenchymal stromal cells (MSC), tumor infiltrating lymphocytes (TIL) and dendritic cells (DCs) for clinical trials.
  • Selection and culturing of CD34+ cells and MK differentiation assays
  • Lentiviral transduction
  • Flowcytometry
  • qPCR
2010-presentGroupleader Megakaryopoiesis, Department of Hematopoiesis, Sanquin Research
2010-presentProject leader Cellular Therapies, Laboratory for Cell Therapy, Sanquin Research 
2004-2010Postdoctoral fellow at Dept of Experimental Immunohematology, Sanquin Research, Amsterdam
2000-2004PhD at Dept of Endocrinology and Metabolism, Academic Medical Centre, Amsterdam

Other academic activities

2011-presentRepresentative of ISBT in Cellular Therapy Coding and labeling Advisory group
Sanquin publications
Other publications

Thijssen-Timmer DC, Peeters RP, Wouters P, Weekers F, Visser TJ, Fliers E, Wiersinga WM, Bakker O, Berghe GV. Thyroid hormone receptor isoform expression in livers of critically ill patients. Thyroid 2007; 17(2):105-12.

Timmer DC, Bakker O, Wiersinga WM, Triiodothyronine affects the alternative splicing of thyroid hormone receptor alpha mRNA. J Endocrinology 2003; 179(2), 217-25.

Boelen A, Kwakkel J, Thijssen-Timmer DC, Alkemade A, Fliers E, Wiersinga WM. Simultaneous changes in central and peripheral components of the hypothalamus-pituitary-thyroid axis in lipopolysaccharide-induced acute illness in mice. J Endocrinology 2004; 182(2):315-23.

Bakker O, Thijssen-Timmer DC, Housekeeping genes: A gold standard? Rapid Cycle Real-time PCR, Methods and Applications, 2004 3-10, Springer-Verlag, ISBN 3-540-20629-9.

Ramakers C, Thijssen-Timmer DC, Bakker O, Lekanne Deprez RH, Ruijter J, Moorman AFM, Quantification of mRNA using linear regression of log-linear PCR data-points as an alternative for the standard curve approach. Rapid Cycle Real-time PCR, Methods and Applications, 2004 21-29, Springer-Verlag, ISBN 3-540-20629-9.

Thijssen-Timmer DC, Schiphorst MP, Kwakkel J, Emter R, Kralli A, Wiersinga WM, Bakker O. PGC-1{alpha} regulates the isoform mRNA ratio of the alternatively spliced thyroid hormone receptor {alpha} transcript. J Mol Endocrinol. 2006; 37(2):251-7.

Last edited on: 10 January 2014
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