Coert (C) Margadant PhD
- Molecular Cell Biology
Molecular Cell Biology
- Research interests
Integrins are cell-surface receptors that are pivotal for human health, and defects in integrins or integrin-associated proteins underlie a variety of pathological conditions including skin blistering, kidney failure, fibrosis, cancer, immunodeficiency, and bleeding disorders. It is therefore essential to tightly control integrin expression and activation, as well as their dynamic regulation by endo-exocytic shuttling (trafficking). My research focuses on how integrin function is regulated, and how the transport machinery of the cell coordinates trafficking of integrins and other cell-surface receptors to dictate cell behaviour.
- Live-cell microscopy
- Cell biological and biochemical techniques
After obtaining my degree in Molecular Cell Biology from Utrecht University, I have been working at several laboratories including Cell Biology (Utrecht University, The Netherlands), the Hubrecht Institute for Developmental Biology and Stem Cell Research (Utrecht, The Netherlands), the Beatson Institute for Cancer Research (Glasgow, UK), and the Netherlands Cancer Institute (Amsterdam, The Netherlands). I received my PhD-degree (cum laude) from the University of Amsterdam for my work on integrin function and trafficking.
- Sanquin publications
- Other publications
Gautrot JE, Malmström J, Sundh M, Margadant C, Sonnenberg A, Sutherland DS.The nanoscale geometrical maturation of focal adhesions controls stem cell differentiation and mechanotransduction. Nano Lett 2014; 14(7):3945-52.
Margadant C, Kreft M, Zambruno G, Sonnenberg A. Kindlin-1 regulates integrin dynamics and adhesion turnover. PLOS ONE 2013; 8(6):e65341.
Postel R*, Margadant C*, Kreft M, Janssen H, Secades P, Zambruno G, Sonnenberg A. Kindlin-1 mutant zebrafish as an in vivo model sytem to study adhesion mechanisms in the epidermis. J Invest Dermatol 2013; 133(9):2180-90.
Margadant C, Cremers L, Sonnenberg A, Boonstra J. MAPK uncouples cell cycle progression from cell spreading and cytoskeletal organization in cycling cells. Cell Mol Life Sci 2013; 70(2):293-307.
Margadant C*, van den Bout I*, van Boxtel AL*, Thijssen VL, Sonnenberg A. Epigenetic regulation of galectin-3 expression by ß1 integrins promotes cell adhesion and migration. J Biol Chem 2012; 287(53):44684-93.
Nicolaou N*, Margadant C*, Kevelam SH, Lilien MR, Oosterveld MJS, Kreft M, van Eerde AM, Pfundt R, Terhal PA, van der Zwaag B, Nikkels PGJ, Sachs N, Goldschmeding R, Knoers NV, Renkema KY, Sonnenberg A. Gain-of-glycosylation in integrin a3b1 causes lung disease and nephrotic syndrome. J Clin Invest 2012; 122(12):4375-87.
Margadant C, Kreft M, de Groot DJ, Norman JC, Sonnenberg A. Distinct roles of talin and kindlin in regulating integrin a5b1 function and trafficking. Curr Biol 2012; 22(17):1-10.
Margadant C, Monsuur HN, Norman JC, Sonnenberg A. Mechanisms of integrin activation and trafficking. Curr Opin Cell Biol 2011; 23(5):607-14.
Margadant C, Charafeddine RA, Sonnenberg A. Unique and redundant functions of integrins in the epidermis. FASEB J 2010; 24(11):4133-52.
Margadant C, Sonnenberg A. Integrin-TGF-b crosstalk in fibrosis, cancer and wound healing. EMBO Rep 2010; 11(2):97-105.
Margadant C, Raymond K, Kreft M, Sachs N, Janssen H, Sonnenberg A. Integrin a3b1 inhibits directional migration and wound re-epithelialization in the skin. J Cell Sci 2009; 122(Pt 2):278-88.
Margadant C, Frijns E, Wilhelmsen K, Sonnenberg A. Regulation of hemidesmosome disassembly by growth factor receptors. Curr Opin Cell Biol 2008; 20(5):589-96.
Wilhelmsen K, Litjens SH, Kuikman I, Margadant C, van Rheenen J, Sonnenberg A. Serine phosphorylation of the integrin b4-subunit is necessary for epidermal growth factor receptor-induced hemidesmosome disruption. Mol Biol Cell 2007; 18(9):3512-22.
Margadant C, van Opstal A, Boonstra J. Focal adhesion signaling and actin stress fibers are dispensable for progression through the ongoing cell cycle. J Cell Sci 2007; 120(Pt 1):66-76.
van Opstal A, Bijvelt JJ, Margadant C, Boonstra J. Role of signal transduction and actin in G1 phase progression. Adv Enzyme Regul 2005; 45:186-200.