Anja (JA) ten Brinke PhD
Biochemistry and Cell biology
'Palmitoylation of the surfactant protein C precursor'
University of Utrecht.
- Research interests
Immune modulation and Immunomonitoring
Dendritic cells (DCs) are the most important antigen presenting cells in our body and are able to direct the development of immune responses. Therefore the application of ex vivo generated DCs has potential to treat diseases which need activation of the immune system, e.g. cancer or chronic infections, or to inhibit immune reactions which are unwanted, e.g. in autoimmunity or transplantation. We developed protocols for the generation of clinical applicable activating DCs and tolerogenic DCs.
The focus of our research is the modulation of immune responses by DCs. Currently, we are exploring the effect of complement anaphylatoxin C5a on DC function, such as cytokine production, antigen presentation and T cell polarization. Besides we study the regulation of DC function by platelets, since platelets are increasingly acknowledged for their role in modulating immune responses.
Furthermore we are interested in monitoring the effects of immunomodulatory therapies on the immune system. The characterization of CD4+ T cells is crucial for understanding their role in immunotherapeutic approaches such as vaccinations or tolerizing therapies. Currently we are developing assays to monitor antigen-specific CD4 T cell responses in a detailed manner.
- Cell culture
- Multi-colour Flow cytometry
- T cell assays
- Migration analysis
- Biochemical analysis
2012-present Group leader at Dept of Immunopathology, subgroup Immune modulation at Sanquin Research, Amsterdam 2008-2012 Senior scientst at Dept of Immunopathology, DC group at Sanquin Research, Amsterdam 2004-2008 Post-doc at Dept of Immunopathology, subgroup Autoimmune Regulation at Sanquin Research, Amsterdam 2002-2004 Post-doc at the Dept of Immunopathology, subgroup Autoimmune diseases at Sanquin Research, Amsterdam 1997-2002 PhD student at Dept of Biochemistry and Cell Biology, faculty of Veterinary Medicine, University of Utrecht 1992-1997 Study Medical Biology at the University of Utrecht
- Sanquin publications
- Other publications
Ten Brinke A, Posthuma G, Batenburg JJ, Haagsman HP, Ridder ANJA, van Golde LMG and Vaandrager AB. The transmembrane domain of surfactant protein C precursor determines the morphology of the induced membrane compartment in CHO cells. Eur J Cell Biol 2003 82(6):285-94.
Brasch F, Ten Brinke A, Johnen G, Ochs M, Kapp N, Müller KM, Beers MF, Fehrenbach H, Richter J, Batenburg JJ and Bühling F. Processing of the hydrophobic surfactant associated protein C in type II cells of the human lung. Am J Respir Cell Mol Biol 2002; 26(6):659-70.
Ten Brinke A, van Golde LMG, Batenburg JJ. Palmitoylation and processing of the lipopeptide surfactant protein C. Review in Biochim Biophys Acta 2002; 1583(3):253-65.
Ten Brinke A, Batenburg JJ, Haagsman HP, van Golde LMG, Vaandrager AB. Differential effect of brefeldin A on the palmitoylation of surfactant protein C proprotein mutants. Biochem Biophys Res Commun 2002; 290(1):532-8.
Ten Brinke A, Vaandrager AB, Haagsman HP, Ridder ANJA, van Golde LMG, Batenburg JJ. Structural requirements for palmitoylation of surfactant protein C precursor. Biochem J 2002; 361(Pt 3):663-71.
Ten Brinke A, Batenburg JJ, Gadella BM, Haagsman HP, Vaandrager AB, van Golde LMG. The juxtamembrane lysine and arginine residues of surfactant protein C precursor influence palmitoylation via effects on trafficking. Am J Respir Cell Mol Biol 2001; 25(2):156-63.
Fry BN, Korolik V, ten Brinke A, Pennings MT, Zalm R, Teunis BJ, Coloe PJ, van der Zeijst BA. The lipopolysaccharide biosynthesis locus of Campylobacter jejuni 81116. Microbiology 1998: 144:2049-61.