Thesis Ammarina Beumer-Chuwonpad

On 24 October 2023 Sanquin researcher Ammarina Beumer-Chuwonpad defended her thesis 'The road to residency: Uncovering the proteome, metabolism and transcriptional networks of tissue-resident memory T cells' at the University of Amsterdam.

Promotor: Prof RAW van Lier MD PhD
Copromotor: KPJM van Gisbergen PhD
Venue: Agnietenkapel, University of Amsterdam

Summary

CD8+ memory T cells persist after infection as circulating and resident subsets to increase the capacity of the immune system to eradicate viruses and other intracellular pathogens upon secondary encounter. The ability of memory CD8+ T cells to retain their proliferative potential and to differentiate into effectors are characteristics that can be exploited for their in vitro expansion into advanced medicinal cell-based products for adoptive T cell therapies. Improving T cell centered immunotherapy requires more knowledge regarding the differentiation potential, metabolic wiring and transcriptional regulation of distinct subsets of memory T cells. Therefore, in this thesis, we explored the in vivo and in vitro reactivation of circulating and resident memory CD8+ T populations and focused on their differentiation pathways, relevant environmental cues, and important metabolic and transcriptional regulators.

Chapters

Chapter 1
General introduction: Transcriptional regulation of tissue-resident memory CD8+ T cells

Chapter 2
Circulating memory CD8+ T cells are limited in forming CD103+ tissue-resident memory T cells at mucosal sites after reinfection abstract

Chapter 3
Memory CD8+ T cells upregulate glycolysis and effector functions under limiting oxygen conditions abstract

Chapter 4
Tissue-resident memory T cells maintain distinct identity from circulating memory T cells after in vitro restimulation

Chapter 5
Tissue-resident memory T cells require mTORC1 for their formation, but not for their maintenance

Chapter 6
Hobit and Blimp1 regulate TRM abundance after LCMV infection by suppressing TRM precursor tissue exit abstract

Chapter 7
General discussion: TRM potential for adoptive immunotherapy against cancer

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Download PhD thesis (university repository)

Thesis Ammarina Beumer-Chuwonpad