Donor InSight-III almost ready to analyse
Donor InSight (DIS)-III data collection will be completed soon! We expect to have new data on approximately 3,000 DIS-donors by the end of this year. From these donors, blood samples were collected, questionnaires retrieved, and -in a subset of participating donors- accelerometers were used to objectively measure physical activity.
DIS-III data-collection started in April 2015 and ends in November 2016. We invited donors who previously participated in DIS-I and/or DIS-II. This population also includes donors who already stopped donating, to not introduce potential selection bias. We have sent around 6,000 invitation letters to reach a response of approximately 3,000 donors.
Blood samples were collected from donors across the Netherlands. This means that blood samples were collected at all collection sites of Sanquin, including mobile locations. The locations sent their collected blood samples to Amsterdam. We processed the samples the next morning at the National Screening laboratory of Sanquin (NSS).
During the busiest DIS-days we received up to 60 blood samples per week (Tuesday to Saturday). We collected four tubes per donor; one tube for the full blood counts (Sysmex) and hemolysis assessments at Sanquin’s department of Product and Process Development (PPO) in Amsterdam, one tube for DNA isolation at the Molecular Platform in Amsterdam (isolated DNA will be sent to the U.K. for quality control and preparation for the SNP array), one tube for zinc protoporphyrin (ZPP) measurements was sent to the Quality Control laboratory at Sanquin Nijmegen and one tube stayed at the NSS for measuring ferritin and sample storage.
Apart from blood sampling, questionnaires were also retrieved from participating donors. Each donor was asked to answer questions regarding lifestyle (International physical activity questionnaire (IPAQ), short version of Food Frequency Questionnaire (FFQ), smoking, alcohol use and medication use) and health status (SF-12 for general health, restless legs syndrome, cognitive functioning, pica, and fatigue). Female donors were also asked to complete questionnaires on menstruation (including a pictorial blood loss assessment chart (PBAC)), menopause and pregnancies. Physical activity was more accurately assessed by having a subset (n≈750) of the DIS-III participants wearing a physical activity monitor (accelerometer).
Data cleaning for DIS-III has already started and DNA samples will be analyzed in 2017. Soon we will have a large dataset containing a lot of information on the participating donors. This offers ample possibilities for future studies. Tiffany Timmer and Rosa de Groot have put a lot of effort into making this project a big success and will soon start working on the very first analyses. Please visit this webpage for more information on the exact DIS-III measurements.