Thesis Nathalie Lak
On 19 March 2024 Sanquin and PMC researcher Nathalie Lak defended her thesis 'Liquid biopsies in pediatric rhabdomyosarcoma and beyond' at Utrecht University.
Promotores
Prof MM van Noesel MD PhD and
Prof CE van der Schoot MD PhD
Co-promotores
GAM Tytgat MD PhD
J Stutterheim PhD
Venue
Academy Building, Utrecht University
Summary
In the Netherlands, more than 500 children are diagnosed each year with a pediatric malignancy, of which 33% with a solid tumor. Treatment for these solid tumors is intensive and has a great impact on quality of life. Additionally, it is hard to identify patients upfront with therapy resistant disease which can lead to recurrent disease and is associated to high mortality.
Currently, treatment stratification and response monitoring is based on analysis of tumor material and imaging studies, which can be a burden for the patients and have limited sensitivity. Minimally invasive sampling of blood to detect circulating tumor material using molecular analysis, i.e. liquid biopsies, can form an additional modality to improve current risk stratification at diagnosis and response monitoring during treatment. In the first part of this thesis, we demonstrated that presence of tumor-specific material in small volumes of blood from patients with rhabdomyosarcoma (tumor of the muscles) can predict clinical outcome. Furthermore, we showed that it is feasible to design patient-specific assays suitable for clinical application and different liquid biopsy-based techniques can be complementary.
In the second part of this thesis, we also investigated further novel molecular techniques for liquid biopsy analysis of patients with different types of solid tumors, such as neuroblastoma and Ewing sarcoma.
In conclusion, analysis of liquid biopsies can be of added value to current clinical methods and ultimately help to improve outcome for children with solid tumors.
Chapters
Chapter 1
lntroduction and outline of this thesis
Part 1: liquid biopsies in pediatric rhabdomyosarcoma
Chapter 2
Improving Risk Stratification for Pediatric Patients with Rhabdomyosarcoma by Molecular Detection of Disseminated Disease abstract
Chapter 3
Novel Circulating Hypermethylated RASSF1A ddPCR lor liquid Biopsies in Patients With Pediatric Solid Tumors abstract
Chapter 4
Cell-free DNA as a diagnostic and prognostic biomarker in pediatric rhabdomyosarcoma abstract
Chapter 5
Molecular Characterization of Circulating Tumor DNA in Pediatric Rhabdomyosarcoma: A Feasibility Study abstract
Chapter 6
Targeted Locus Amplification to develop robust patientspecific assays for liquid biopsies In pediatric solid tumors abstract
Part 2: Extracellular vesicles and cell-free RNA
Chapter 7
Extracellular Vesicles: A New Source of Biomarkers in Pediatric Solid Tumors? A Systematic Review abstract
Chapter 8
Cell-free RNA from plasma in patients with neuroblastoma: exploring the technical and clinical potential abstract
Chapter 9
Discussion and future directions
Details
Download PhD thesis (university repository)
