Back to the overview page

Sulima (SC) Geerman

Department
Hematopoiesis
E-mail
s.geerman@sanquin.nl
Training

BSc in Biomedical Research, Noordelijke Hogeschool Leeuwarden and MSc in Biomedical Sciences, University of Groningen.

Research interests
The impact of immune activation on hematopoiesis
Technology
  • Flow cytometry
  • Cell sorting
  • Transplantation (mice)
  • Transwell migration assay
  • Proteomics
  • ELISA
  • Cell culture
Resume
2010-2011Student researcher at University Medical Center Groningen, Department of Cell Biology, Stem Cell Biology supervised by Dr. Brad Dykstra and Prof. Gerald de Haan
Title: 'Rainbow” stem cell transplants to study changes in the hematopoietic stem cell pool'
Techniques: Harvesting of bones (mice), isolation of bone marrow cells, flow cytometry (9 colors), cell sorting, transplantations in mice and migration assays
Bioinformatic tool: FlowJo
2010Student researcher at University Medical Center Groningen, Department of Pathology and Medical Biology supervised by Dr. Lydia Visser and Prof. Anke van den Berg
Title: 'The tumor microenvironment in Hodgkin lymphoma: Crosstalk between HRS cells and T cells'
Techniques: Culturing of tumor cells, isolation and analysis of HRS cell secretome and membrane proteins (LC-MS/MS), ELISA and immunohistochemistry.
Bioinformatic tools: UniProtKB and DAVID (EASE)
2008Student researcher at University Medical Center Groningen, Center of Medical Biomics - Proteomics Facility supervised by Dr. Han Roelofsen
Title: 'Proteomics analysis of the effect of leptin on human macrophages'
Techniques: Generation and culturing of human macrophages, SILAC, LC-MS/MS sample preparation (fractionation, tripsin digestion, peptide extraction) and multiplex cytokine assay.
Bioinformatic tools: KEGG, AmiGO and STRING
2007-2008Student Medical Researcher at Medical Center Leeuwarden, Klinisch Chemisch Laboratorium supervised by Dr. Rebecca Fokkema and Dhr. Rudi de Jong
Title: 'Evaluation of the Lupus Anticoagulant test on the ACL-TOP'
Techniques: Venopuncture, finger puncture, safe handling of human biomaterials, general diagnostic laboratory work.
Publications
Sanquin publications
Last edited on: 20 September 2013
Back to the overview page