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Ruchira (R) Engel-Probodh PhD

Department
Immunopathology
E-mail
r.engel@sanquin.nl
LinkedIn
http://nl.linkedin.com/in/ruchiraengel
Training

Physical Chemistry

Thesis

‘Dictyostelium chemotaxis studied with fluorescence fluctuation spectroscopy’
September 2005 - Wageningen University

Research interests
C1-inhibitor My research is focussed on elucidating the role of the N-terminal domain of C1-inhibitor (C1-inh) in anti-inflammation. I am particularly interested in the effect of glycosylation of the N-terminal domain. C1-inh is an anti-inflammatory plasma protein and is the major inhibitor of proteases of the complement and contact pathways. Recent in vivo studies have shown that C1-inh can also have anti-inflammatory properties independent of its protease inhibitory functions. These novel properties of C1-inh are attributed to the glycosylations on the non-protease N-terminal domain of the protein. Specifically, I am studying the anti-inflammatory effects of C1-inh mediated by interactions with endothelial cell surface proteins and with lipopolysaccharides. In addition, I am interested in the changes in C1-inh glycosylation during inflammation.
Technology
  • ELISA
  • Enzyme kinetics assays (chromogenic assays)
  • Gel electrophoresis
  • Western blot
  • Fluorescence spectroscopy
  • Confocal microscopy
  • Surface plasmon resonance
Resume
2010-presentPostdoctoral researcher Dept of Immunopathology, Sanquin  Research
2007-2010Research Fellow Faculty of Biological Sciences, University of Leeds (UK)
2005-2007Postdoctoral researcher Laboratory of Biochemistry, Wageningen University
2000-2005PhD Biochemistry, Wageningen University, The Netherlands
1999-2000Research Officer, Unilever Research India, Bangalore, India
1997-1999MSc Physical Chemistry, University of Pune, India
Publications
Sanquin publications
Other publications

Engel R, Brain CM, Paget J, Lionikiene AS, and Mutch NJ. Single-chain factor XII exhibits activity when complexed to polyphosphate. J Thromb Haemost 2014;12(9):1513-22.

Laptenok SP, Visser NV, Engel R, Westphal AH, van Hoek A, van Mierlo CP, van Stokkum IH, van Amerongen H, Visser AJ. A General Approach for Detecting Folding Intermediates from Steady-State and Time-Resolved Fluorescence of Single-Tryptophan-Containing Proteins. Biochemistry 2011; 50(17):3441-50.

 

Skakun VV, Engel R, Digris AV, Borst JW, Visser AJ. Global analysis of autocorrelation functions and photon counting distributions. Front Biosci (Elite Ed). 2011; 3:489-505.

Mutch NJ, Engel R, Uitte de Willige S, Philippou H, Ariëns RA. Polyphosphate modifies the fibrin network and down-regulates fibrinolysis by attenuating binding of tPA and plasminogen to fibrin. Blood 2010; 115(19):3980-8.

Engel R, Westphal AH, Huberts DH, Nabuurs SM, Lindhoud S, Visser AJ, van Mierlo CP. Macromolecular crowding compacts unfolded apoflavodoxin and causes severe aggregation of the off-pathway intermediate during apoflavodoxin folding. J Biol Chem 2008; 283(41):27383-94.

Aker J, Hesselink R, Engel R, Karlova R, Borst JW, Visser AJ, de Vries SC. In vivo hexamerization and characterization of the Arabidopsis AAA ATPase CDC48A complex using forster resonance energy transfer-fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy. Plant Physiol 2007; 145(2):339-50.

Engel R, Van Haastert PJ, Visser AJ. Spectral characterization of Dictyostelium autofluorescence. Microsc Res Tech 2006; 69(3):168-74.

Knol JC*, Engel R*, Blaauw M, Visser AJ, van Haastert PJ. The phosducin-like protein PhLP1 is essential for G{beta}{gamma} dimer formation in Dictyostelium discoideum. Mol Cell Biol 2005; 25(18):8393-400. (*Equal contribution)

Skakun VV, Hink MA, Digris AV, Engel R, Novikov EG, Apanasovich VV, Visser AJ. Global analysis of fluorescence fluctuation data. Eur Biophys J 2005; 34(4):323-34. Erratum in: Eur Biophys J 2005; 34(7):972.

Bosgraaf L, Waijer A, Engel R, Visser AJ, Wessels D, Soll D, van Haastert PJ. RasGEF-containing proteins GbpC and GbpD have differential effects on cell polarity and chemotaxis in Dictyostelium. J Cell Sci 2005, 118(Pt 9):1899-910.

Veltman DM, Roelofs J, Engel R, Visser AJ, Van Haastert PJ. Activation of soluble guanylyl cyclase at the leading edge during Dictyostelium chemotaxis. Mol Biol Cell 2005; 16(2):976-83.

Ruchira, Hink MA, Bosgraaf L, van Haastert PJ, Visser AJ. Pleckstrin homology domain diffusion in Dictyostelium cytoplasm studied using fluorescence correlation spectroscopy. J Biol Chem 2004; 279(11):10013-9.

Blaauw M, Knol JC, Kortholt A, Roelofs J, Ruchira, Postma M, Visser AJ, van Haastert PJ. Phosducin-like proteins in Dictyostelium discoideum: implications for the phosducin family of proteins. EMBO J 2003; 22(19):5047-57.

Last edited on: 7 October 2015
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