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Jesse (J) Eernstman

Department
Hematopoiesis
E-mail
j.eernstman@sanquin.nl
LinkedIn
http://www.linkedin.com/pub/jesse-eernstman/52/522/463
Training

MSc Medical Biology (Radboud University Nijmegen)

Research interests
The aim of our research is to culture erythrocytes from induced pluripotent stem cells (iPS) with specific blood group genotypes, to reduce donor dependency and prevent allo-immunisation. To establish human iPS we transduce erythroblasts with a polycistronic lentiviral vector expressing Oct4, Sox2, c-Myc, and Klf4. The transduced cells were transferred to a MEF feeder culture and iPS medium. Embryoid bodies can be generated for which the iPS cells were transferred to uncoated plates in medium supplemented with BMP4, VEGF, SCF, TPO, IL-6, IL-3, insulin, and cholesterol rich lipids. After 14 days embryoid bodies generated from erythroblast-derived iPS contained blood islands that were visible because of the presence of red cells. The next challenge is the large scale expansion of iPS-derived erythroblasts and subsequent maturation to hemoglobinised, enucleated red blood cells.
Technology
  • Flow Cytometry
  • Cell Culturing
  • Transfection
  • Transduction
  • Cloning
  • qPCR
  • Western Blotting
  • Mass-spec
  • HPLC
Resume
2013-present PhD student at the Dept of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, Amsterdam
2011 ServiceXS, Leiden, Traineeship:
Epigenetics; protocol development methylation analysis using bisulfite conversion. Innovation studies of cytosine methylation and 5-hydroxymethylation
2010

Erasmus MC, Rotterdam, Cell Biology, Diploma/Master thesis: The regulation and involvement of the proteins A disintegrin and metalloproteinase domain 17 (ADAM17) and Interleuking-6 receptor alpha (IL6r) in Cystic Fibrosis (CF) and down-regulation of the epithelial sodium channel (ENaC) in CF primary bronchial epithelial cells by lentiviral shRNA transduction, to monitor basal fluid resorption.

Scope: Cell biological investigation of machinery in Cystic fibrosis-primary bronchial epithelial cells by high-resolution fluorescent microscopy, ELISA and measuring net ion transports

2010-2012 MSc degree in Medical Biology, Radboud University Nijmegen
2009 Occupational Trainee, University of Queensland, Australia
Bachelor thesis: Understanding the effect of ABA (Abscisic acid) on growth of the transformed legume Lotus japonicas.
2005-2009 Bachelor in Biochemistry, HAN University Nijmegen
Publications
Sanquin publications
Last edited on: 29 October 2014
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