Jeffrey (J) Kroon
- Department
- Molecular Cell Biology
- j.kroon@sanquin.nl
- http://nl.linkedin.com/pub/jeffrey-kroon/24/a2b/114
- Training
Cell biology/Biochemistry
- Research interests
- Endothelial cells lining the lumen of arteries are constantly subjected to a high pulsatile laminar flow and high mechanical shear stress. This high shear stress plays an important role in vascular remodeling and has an atheroprotective function, whereas turbulent flow associated with low shear stress is related with the on-set of diseases such as atherosclerosis. Therefore, it is important to understand how endothelial cells sense the changes in physiological shear stress and adapt in response to flow. A key-event in response to flow is re-arrangement of the actin cytoskeleton. It is known that Rho GTPases play a pivotal role in this process. Our current focus is identifying the pathways involved in Rho GTPase activation and its upstream activators under physiological flow conditions.
- Technology
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- In vitro flow assays
- Pulldown and immunoprecipitation assays for analysis of protein-protein interactions
- Confocal microscopy and live cell imaging
- Cell culturing
- Western blotting
- Resume
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2010-heden PhD-student at the Dept of Molecular Cell Biology, Sanquin Research, Amsterdam 2010 MSc in Biomolecular Sciences at the VU University, Amsterdam 2010 Internship at the Department of Genetics & Pathology: Vascular Biology, Uppsala University, Uppsala, Sweden 2009 Internship at the department of Oral Microbiology, Academic Centre for Dentistry, Amsterdam 2008 BSc in Biochemistry, INHolland, Alkmaar 2007-2008 Internship at the Dept of Molecular Cell Biology, Sanquin Research, Amsterdam 2006 Internship at the Dept of Molecular Cell Biology and Immunology, VU Medical Centre (VUmc), Amsterdam - Publications
- Sanquin publications
- Other publications
van der Bij GJ, Bögels M, Oosterling SJ, Kroon J, Schuckmann DT, de Vries HE, Meijer S, Beelen RH, van Egmond M. Tumor infiltrating macrophages reduce development of peritoneal colorectal carcinoma metastases. Cancer Lett 2008; 262(1):77-86.