Janny (J) de Wildt-Eggen PhD
- Department
- Transfusion Monitoring
- j.dewildt@sanquin.nl
- http://www.linkedin.com/pub/janny-de-wildt/26/4aa/75a
- Thesis
'New development in the preparation and storage of platelet concentrates from buffy coats'
2003 - University of Utrecht- Research interests
- Proficiency testing Yearly organization of 5 national proficiency studies: Platelet counts in platelet concentrates; leucocyte counts in blood products; pH, blood gas, and metabolite measurements in platelet concentrates; CD62p and Annexin V expression in platelet concentrates; trombocyte count using CD41 in platelets concentrate. Product development Cryoseal (in Dutch called cryolijm) is a fibrin glue of single donor plasma which Sanquin has started to produce as an alternative for autologous or pooled plasma fibrin glues. Studies have been performed to elongate the storage time. Furthermore validation studies are ongoing to produce cryoseal from whole blood derived plasma and to produce cryoseal with a longer clotting time to broaden the use of cryoseal. Cryo-poor plasma, a by product of cryoseal production, might be a possible therapy for patients with TTP. A Hemotopoietic Progenitor Cell (HPC)-simulant has been developed which can serve as a substitute for patient’s material to be used for validation studies. Relation between in vivo and in vitro parameters During a period of 1.5 years all platelet concentrates were prepared in a BCSI container. This storage bag enables pH measurement at any time during storage without sampling, i.e. in a sterile way. The pH of the platelets concentrates was studied to determine whether or not there is a correlation with the in vitro quality and/or bacterial contamination, storage time and the yield of the transfusion in patients.
- Resume
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2011-present Manager Dept Transfusion Monitoring at Sanquin Research and Manager Production at Sanquin Blood Supply North East Region, Groningen 2008-201 Manager Laboratory IHD, Manager Research & Education and Manager Production at Sanquin Blood Supply North East Region, Groningen 2007-present Processing Facility Director (JACIE) at Sanquin Blood Supply North East Region, Groningen 2007-2008 Manager Laboratory, Manager Production and Manager Research & Education at Sanquin Blood Bank North East Region, Groningen 2002-2007 Manager Laboratory at Sanquin Blood Bank North East Region, Groningen 2004-2005 Manager Quality Assurance a.i. at Sanquin Blood Bank North East Region, Groningen 1988-2010 Teacher immunology and blood group systems at the Blood bank Assistant course 2000-2002 Laboratory Manager and product development at Blood bank IJssellanden, Zwolle 1989-2000 Teacher bloodgroup systems (Kell, Duffy, Kidd, Lutheran, Lewis, I,P system) at Hogeschool Gelderland, Nijmegen 1990-2000 Head of the Laboratory, Head of the Department of Component Preparation and Research and Development at Blood Bank, Zwolle 1985-1990 Head of the laboratory, head of the department of component preparation at Blood Bank, Zwolle 1978-1985 Head of the Laboratory, Blood Bank, Zwolle - Publications
- Sanquin publications