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Farzin (F) Pourfarzad

Blood Cell Research

Molecular Biology/Microbiology
Human Genetics


Human gammaglobin gene re-activation to treat thalassemia and sickle cell anemia
Erasmus Medical Centre, Rotterdam

Research interests
Chromatin Biology, transcription and epigenetic regulation during hematopoiesis differentiation

Molecular biology; Conventional and advanced molecular biology, cloning, blotting, hybridisation, radioactive labelling, PCR, microarray, chromatin pull down, sequencing, lentiviral gene transfer, RNAi, etc.
Proteomics; Protein purification, detection, immunoprecipitation, epitope tagging, mass spectrometry.
Cell biology; Mammalian cell lines and human primary cell culture
Animal models; Transgenic, knock out and congenic mouse and rat models.
Human genetic prenatal and molecular diagnosis; Cytogenetics, mutation detection, linkage analysis, QTL mapping

2013-presentPost-doc, Dept of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam.
Project: Epigenetic regulation of neutrophil granulocyte differentiation
2009-2012Post-doc: Medical Genetic Cluster, Dep of Cell Biology and Genetics, Erasmus Medical Centre, Rotterdam.
Project: The role of the ZBP-89 protein complex in globin gene regulation
2001-2002Research assistant, Dept of Human Genetics, Leiden University Medical Centre
1999-2001Molecular genetic laboratory, Genetic Research Centre, Welfare and Rehabilitation University, Tehran, Iran
1999-2001CEO/Owner 'Bio-Research alphabet' consultancy for diagnostic molecular biology laboratories set up, molecular biology Service Company, and oligonucleotides synthesis in Iran
1997-2001Molecular Prenatal Diagnosis of common hereditary diseases in Iran (e.g. Thalassemia, DMD/BMD, SMA), Genetic and Pathology Centre, Tehran, Iran
1996-1998MSc, Human Genetics,Tehran University of Medical Sciences, Iran.
Project: Rb gene mutation detection in Retinoblastoma patients by PCR-SSCP
1992-1996BSc, Molecular Biology/Microbiology, Cum Laude, Zanjan Free University, Iran
Sanquin publications
Other publications

Pourfarzad F, von Lindern M, Azarkeivan A, Hou J, Kheradmand KS, Esteghamat F, van IJcken W, Philipsen S, Najmabadi H and Grosveld F. Hydroxyurea responsiveness in beta-thalassemic patients is determined by the stress response adaptation of erythroid progenitors and their differentiation propensity. Haematologica 2012 Dec 14.

Kheradmand KS, Verbeek E, Engelen E, Schot R, Poot RA, de Coo IF, Lequin MH, Poulton CJ, Pourfarzad F, Grosveld FG, Brehm A, de Wit MC, Oegema R, Dobyns WB, Verheijen FW, and Mancini GM. RTTN mutations link primary cilia function to organization of the human cerebral cortex. Am J Hum Genet 2012; 91(3):533-40.

Fanis P, Gillemans N, Aghajanirefah A, Pourfarzad F, Demmers J, Esteghamat F, Vadlamudi RK, Grosveld F, Philipsen S and van Dijk TB. Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation. Mol Cell Proteomics 2012; 11(11):1263-73.

Banan M, Esmaeilzadeh-Gharehdaghi E, Nezami M, Deilami Z, Farashi S Philipsen S, Esteghamat F, Pourfarzad F, Ali Imam AM and Najmabadi H. cAMP response element-binding protein 1 is required for hydroxyurea-mediated induction of gamma-globin expression in K562 cells. Clin Exp Pharmacol Physiol 2012; 39(6):510-7.

Esteghamat F, van Dijk TB, Braun H, Dekker S, van der Linden R, Hou J, Fanis P, Demmers J, van IJcken W, Ozgur Z, Horos R, Pourfarzad F, von Lindern M, and Philipsen S. The DNA binding factor Hmg20b is a repressor of erythroid differentiation. Haematologica 2011; 96(9):1252-60.

van Dijk TB, Gillemans N, Pourfarzad F, van Lom K, von Lindern M, Grosveld F and Philipsen S. Fetal globin expression is regulated by Friend of Prmt1. Blood 2010; 116(20):4349-52.

Zukic B, Radmilovic M, Stojiljkovic M, Tosic N, Pourfarzad F, Dokmanovic L, Janic D, Colovic N, Philipsen S, Patrinos GP and Pavlovic S. Functional analysis of the role of the TPMT gene promoter VNTR polymorphism in TPMT gene transcription. Pharmacogenomics 201; 11(4):547-57.

Kheradmand Kia S, Solaimani KP, Farahbakhshian E, Pourfarzad F, von Lindern M, and Verrijzer CP. EZH2-dependent chromatin looping controls INK4a and INK4b, but not ARF, during human progenitor cell differentiation and cellular senescence. Epigenetics Chromatin 2009; 2(1):16.

Kolodziej KE, Pourfarzad F, de Boer E, Krpic S, Grosveld F and Strouboulis J. Optimal use of tandem biotin and V5 tags in ChIP assays. BMC Mol Biol 2009; 10:6.

Chassanidis C, Kalamaras A, Phylactides M, Pourfarzad F, Likousi S, Maroulis V, Papadakis MN, Vamvakopoulos NK, Aleporou-Marinou V, Patrinos GP and Kollia P. The Hellenic type of nondeletional hereditary persistence of fetal hemoglobin results from a novel mutation (g.-109G>T) in the HBG2 gene promoter. Ann Hematol 2009; 88(6):549-55.

Esteghamat F, Imanian H, Azarkeivan A, Pourfarzad F, Almadani N and Najmabadi H. Screening of Iranian thalassemic families for the most common deletions of the beta-globin gene cluster. Hemoglobin 2007;31(4):463-9.

Papachatzopoulou A, Kaimakis P, Pourfarzad F, Menounos PG, Evangelakou P, Kollia P, Grosveld FG and Patrinos GP. Increased gamma-globin gene expression in beta-thalassemia intermedia patients correlates with a mutation in 3'HS1. Am J Hematol 2007;82(11):1005-9.

Kleanthous M, Patsalis PC, Drousiotou A, Motazacker M, Christodoulou K, Cariolou M, Baysal E, Khrizi K, Moghimi B, Pourfarzad F, van Baal S, Deltas C, Najmabadi H and Patrinos GP. The cypriot and Iranian National Mutation Frequency Databases. Hum Mutat 2006; 27(6):598-9.

Grosveld F, Rodriguez P, Meier N, Krpic S, Pourfarzad F, Papadopoulos P, Kolodziej K, Patrinos GP, Hostert A and Strouboulis J. Isolation and characterization of hematopoietic transcription factor complexes by in vivo biotinylation tagging and mass spectrometry. Ann NY Acad Sci 2005; 1054:55-67.

Najmabadi H, Karimi-Nejad R, Sahebjam S, Pourfarzad F, Teimourian S, Sahebjam F, Amirizadeh and Karimi-Nejad MH. The beta-thalassemia mutation spectrum in the Iranian population. Hemoglobin 2001; 25(3):285-96.

Last edited on: 21 April 2016
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