Esther Bloem
- Department
- Plasma Proteins
- e.bloem@sanquin.nl
- Training
Biochemistry
- Research interests
- Structure-function of blood coagulation factor VIII/factor IX
- Technology
-
- Biosensor analysis (BiacoreTM)
- Mass spectrometry
- Enzyme kinetics
- Recombinant protein expression (blood coagulation factors)
- Resume
I have performed my internship at the Netherlands Cancer Institute (NKI), where I searched for inhibitor for deubiquitinating enzymes (DUB). Proteins can be modified by the covalent attachment of ubiquitin on their N-terminus or lysine-residues. Once proteins have four or more ubiquitins attached, they are targeted for degradation by the proteasome. DUBs are capable of taken off these, thereby rescuing ubiquitinated proteins from degradation. In some cancers certain tumor activating proteins are rescued from degradation due to the action of specific DUBs, therefore it would be nice to have specific small molecule inhibitors for these DUBs. Several small molecules were tested for their inhibition potential on four different DUBs and some of these small molecules were identified as (specific) DUB inhibitors.
- Publications
- Sanquin publications