Antje (A) Schaefer PhD
- Department
- Molecular Cell Biology
- a.schaefer@sanquin.nl
- http://nl.linkedin.com/pub/antje-schaefer/4a/4b4/351
- Training
Biochemistry, Biophysical Chemistry, Structural Biology
- Research interests
- Signaling events during transmigration of leukocytes across the vascular endothelium, in particular the functional regulation of the endothelial adhesion molecule ICAM-1, for instance through actin-binding proteins. Leukocyte transmigration has to be strictly regulated to ensure an efficient operation of the immune system.
- Technology
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- Molecular and cell biological methods such as cloning, cell culturing, transfections
- Biochemical and biophysical techniques to analyse protein-protein interactions (with cell lysates and with purified proteins)
- Migration and adhesion assays
- Confocal microscopy and live cell imaging
- Resume
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April 2010-present Postdoc at the Dept of Molecular Cell Biology (group PL Hordijk), Sanquin Research, Amsterdam, The Netherlands 2009-2010 Postdoc at the Dept of Structural Biology (groupA Wittinghofer), Max-Planck-Institue for Molecular Physiology, Dortmund, Germany 2004-2009 PhD student at the Department of Structural Biology (group A Wittinghofer), Max-Planck-Institute for Molecular Physiology, Dortmund, Germany
Thesis: Structural and biochemical analysis of the molecular mechanism of Rho GTPase activating proteins2004 MSc in Biochemistry, University of Bayreuth, Germany
Thesis: Biophysical analysis of the folding mechanism of cold shock proteins (group FX Schmid)2001 BSc in Biochemistry, Ernst-Moritz-Arndt University of Greifswald, Germany 2000 Research Assistant at the Department of Nonlinear Processes in Condensed Matter, Max-Born-Institute for Nonlinear Optics and Short Pulse Spectroscopy, Berlin, Germany
Topic: Isolation and FTIR spectroscopy studies of human myoglobin (group T Elsaesser)Fellowships
2009-2010 Postdoc-fellowship of the Max Planck Society, Germany 2000 Student research fellowship of the Leibniz Association, Germany - Publications
- Sanquin publications
- Other publications
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Schaefer A, Miertzschke M, Berken A, Wittinghofer A. Dimeric plant RhoGAPs are regulated by its CRIB effector motif to stimulate a sequential GTP hydrolysis. J Mol Biol 2011; 411(4):808-22.
Schaefer A, Höhner K, Berken A, Wittinghofer A. The unique plant RhoGAPs are dimeric and contain a CRIB motif required for affinity and specificity towards cognate small G proteins. Biopolymers 2011; 95(6):420-33.
Figure of the structural model was elected for the cover of this issue.Mucha E, Fricke I, Schaefer A, Wittinghofer A, Berken A. Rho proteins of plants – functional cycle and regulation of cytoskeletal dynamics. Eur J Cell Biol 2011; 90(11): 934-43.