News & Hightlights

MCB makes it to the national newspaper

Our recent work shows that the endothelium forms elastic straps around the transmigrating leukocyte in order to limit local vascular leakage.
This work was published in the February issue of Nature Communication.

This was also recognized by 'de Volkskrant' and supported
by an institutional press release.


Headline: annexin a2 regulates icam-1-dependent leukocyte transendothelial migration

Our most recent publication in The Journal of Immunology shows how leukocytes effectively cross the endothelial barrier (Heemskerk et al).
Our paper is being featured by the ‘In This Issue’ section of The Journal of Immunology. ‘In This Issue’ highlights articles considered to be among the top 10% of articles published in the journal. 

Heemskerk N, Asimuddin M, Oort C, van Rijssel J, van Buul JD. Annexin A2 Limits Neutrophil Transendothelial Migration by Organizing the Spatial Distribution of ICAM-1. J Immunol 2016; 196(6):2767-78. Epub 2016 Feb 10.

  Jaap en Niels

Two MCB papers published in the same issue of the Journal of Cell Science

The groups of Mar Fernandez-Borja and of Jaap van Buul have each published their research in the same issue of the Journal of Cell Science.
Jaap van Buul’s research group has investigated the role of the Rac1 GTPase exchange factor Trio in the integrity of the endothelium: Timmerman et al. J Cell Sci 2015;128 (16):3041-54.
The group of Mar Fernandez-Borja reports the role of the cellular prion protein in monocyte adhesion and migration: Richardson et al. J Cell Sci 2015; 128(16): 3018-29.

Both papers were highlighted in the ‘In this issue’ section:
A Trio for Rac1 at endothelial junctions
Prion protein slows down monocytes
In addition, a picture from Timmerman et al was selected for the cover!

   Cover J Cell Sc 2015.w

   2-43-Composite (RGB)_bright.w

Vidi grant for Stephan Huveneers

May 18, 2015 - Sanquin researcher Stephan Huveneers received a Vidi grant for his proposal 'The molecular basis of endothelial mechanotransduction in stiff arteries'. With 800,000 euros from the Dutch Organization for Scientific Research (NWO) he is able to continue to expand his line of research. This prestigious grant offers new perspectives for tresearch into the onset of vascular diseases such as atherosclerosis and will contribute to new treatment options in the future.

Press releasae (in Dutch)

 Stephan Huveneers

In-house seminar prize for Antje Schaefer

This year’s Sanquin's In-house seminar prize was awarded to Antje Schaefer, post-doctoral fellow in the Dept of Molecular Cell Biology. Her oral presentation was judged by all spectators as ‘very clear’, and ‘important fundamental work and yet with clear clinical implications’. Her work shows the importance of the stiffness of the vascular endothelial cells during the process of leukocyte extravasation and was a collaborative effort with the group of Carl Figdor from the Radboud University in Nijmegen. Antje Schaefer

F-actin-anchored focal adhesions distinguish endothelial phenotypes of human arteries and veins

One of our recent publications by Daphne van Geemen et al. made it to the cover of ATVB!
In this paper we present our data of the visualization of focal adhesions and the actin cytoskeleton in human vasculature. A confocal microscopic picture of the endothelium in a human mesenteric artery was selected by the Editors of ATVB for the September 2014 issue.
You may read the scientific story and check out the impressive images we obtained from studying human vessels in the full text version of our manuscript.

Video ‘Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow’

Jeffrey Kroon*, Anna E. Daniel*, Mark Hoogenboezem, Jaap D. van Buul
Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, AMC at University of Amsterdam

Upon inflammation, leukocytes clear invading pathogens. To get to the side of injury, leukocytes first have to cross the vessel wall. This complicated process is very dynamic and requires sophisticated assays to understand the mechanisms that control this process.In the lab we can follow this process in real-time using our dedicated microscopic set-up together with a flow terminal to mimic the bloodstream. We have published this protocol in a new on-line journal that expresses the details of this protocol by a educational video.
This video shows PhD student Jeffrey Kroon, together with Anna Daniel (Dept of Molecular Cell Biology) first author on the paper, explaining the protocol in detail. This assay is of high interest to many labs not only in the Netherlands but worldwide and through this video anyone interested in leukocyte transendothelial migration can copy our set-up to study their specific part of interest of this process.
See film on

Thesis Ilse Timmerman

On 3 July 2013 Ilse Timmerman defended her thesis ‘Dynamic control of VE-cadherin-based junctions’ at the University of Amsterdam.

Promotor: Prof PL Hordijk PhD
Co-promotor: JD van Buul PhD
        Promotie Ilse Timmerman

Thesis Younes Zoughlami

On 25 April 2013 Younes Zoughlami defended his thesis 'New Dimensions in CXCR4 and Rac1 regulation' at the University of Amsterdam.

Promotor: Prof PL Hordijk PhD
Co-promotores: PB van Hennik PhD and C Voermans PhD
       Promotie Younes Zoughlami

Presentation award for Jeffrey Kroon

At the 3rd Cardiovascular Conference (CVC) at Noordwijkerhout, held on 14-15 March 2013, Jeffrey Kroon, PhD student in the group of Jaap van Buul won the presentation award. Jeffrey discussed how endothelial cells that line the lumen of blood vessels align upon the induction of flow. This is important research, since impaired alignment can result in the induction of atherosclerotic plaques. We wish to congratulate Jeffrey with his prize.         Jeffrey Kroon

Thesis Daniël Lam

On 1 March 2013 Daniël Lam defended his thesis ‘Ready, Set? Go! Microscopy and Image Analysis of Cell Signalling in Cell Migration’ at the University of Amsterdam.

Promotor: Prof PL Hordijk PhD
        Promotie Daniel Lam
Last edited on: 12 April 2016