IgG subclass Plus kit for IMMAGE / IMMAGE 800

Division Reagents of Sanquin Blood Supply offers human IgG subclass Plus kit for IMMAGE and IMMAGE 800.

The human IgG subclass Plus kit for IMMAGE / IMMAGE 800 has been designed for fast, reproducible and specific quantification of human IgG subclasses (IgG1, IgG2, IgG3 and IgG4).

Principle

The nephelometric determinations of IgG1 and IgG2 in serum are based on the specific reactions with monospecific, highly avid anti-IgG subclass antisera.

The turbidimetric determinations of IgG3 and IgG4 in serum are based on the specific reaction with a latex reagent coated with the monospecific, highly avid anti-IgG subclass antibodies for greater analytical sensitivity.

The polyclonal reagents in this kit are specific for human IgG subclasses and have been selected for high avidity.

The fractionated sheep anti-human IgG subclass sera are made specific by absorption with isolated gammaglobulins of the unwanted IgG subclasses and, if necessary, by absorption with isolated serum fractions.

Content of the kit

The human IgG subclass Plus kit contains sufficient reagents to measure each of the 4 human subclasses in 50 tests, including calibrators and controls.

Calibrator values

The IgG subclass levels in the calibrators were determined using the ERM-DA470 as calibrator. The assigned values are 6.210 g/L for IgG1, 3.450 g/L for IgG2, 0.390 g/L for IgG3 and 0.591 g/L for IgG4 and are derived from WHO 67/97.

Product number of the kit is M1895.

Last edited on: 22 October 2015