Combinatorial Coding

Division Reagents offers a high-throughput screening of your patient samples to detect antigen specific T cells in vaccination studies or cell therapy.

Limitations current techniques

The detection of antigen-specific CD8+ T cells recognizing an HLA binding peptide is possible by staining these cells with fluorescently labelled multimers. To date, a limited number of antigen-specific T cell populations per sample can be detected.

This limits the ability to perform a comprehensive analysis of disease-associated or therapy-induced T cell responses. In particular for clinical samples this can be a considerable disadvantage.

Advantage of combinatorial coding

Combinatorial coding is a new high-tech method to circumvent the limitation of sample size. With this technique monitoring of T cell recognition of the MHC-peptide complexes can be performed quickly in small sample volumes.

Minimum use of patient sample

28 different MHC/peptide combinations can be analysed simultaneously in one blood sample. Instead of using 200ml of blood, only 8ml of blood is needed to broadly screen for immunogenic peptides.
The combinatorial coding can be used for:

Service testing

Division Reagents of Sanquin Blood Supply offers this high-tech technique as service testing.

Our service testing combines the UV induced peptide exchange technology with the combinatorial coding. In this way the immunogenicity of your eptitopes of interest can be determined fast and efficiently and, high throughput screening of patient samples can be performed to detect antigen specific T cells during clinical interventions.

For more information on the possibilities of service testing, please check our website on service testing or contact us directly at:
T+31 20 512 35 99 
E reagents@sanquin.nl.

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