Retroviruses

XC Plaque Assay 

• Detection of infectious murine ecotropic retroviruses.
• Confirmation of borderline results by means of an immunoassay.

S+L- Focus Assay

• Detection of infectious murine xenotropic retroviruses.
• Confirmation of boderline results by means of an immunoassay.

Reverse Transcriptase Assay

• Assay of pellets in presence of Mg++ or Mn++ to detect human, murine and other retroviruses.
• Concurrent positive and negative controls.
• Confirmation of a positive result is performed by repetition of the assay with two different template-primers in order to discriminate Reverse Transcriptase from cellular DNA polymerase.

XC Plaque Extended Assay

• Subculture with murine detector cells to amplify contaminating virus prior to Assay #BT201.

S+L- Focus Assay

• Subculture with murine detector cells to amplify contaminating virus prior to Assay #BT202.

Mink Cell Focus Assay

• Detection of infectious polytropic murine retroviruses.

Mink Cell Focus Extended Assay

• Subculture with mink detector cells to amplify contaminating virus prior to Assay #BT202.

Co-cultivation Assay with Mus Dunii Cells

• Test is performed in compliance with FDA 'Points to consider'.
• Co-cultivation of test cells with Mus Dunni cells for 5 passages.
• At passage 1 and 5 the cell culture supernatant is tested by means of XC Plaque Assay (#BT 201) and S+L- Focus Assay (#BT202).

Transmission Electron Microscopy (qualitative)

• Detection of viruses in cell cultures by means of examination of 100 cells.

Transmission Electron Microscopy (quantitative)

• Detection and quantification of viruses in cell cultures by means of examination of 200 cells.

Retrovirus Detection and Quantitation by Negative Stain Electron Microscopy

• Detection and quantitation of typical retroviral particles.