Lymphocyte cytokine profiling (ICS)

Monitoring cell populations on the basis of phenotypic markers can provide useful information about immune reactions in patients (Cellular Immunophenotyping (flow cytometry)). Additionally, much more information can be obtained by investigating the functionality of specific immune cells. T cells produce cytokines and/or granzymes upon stimulation with their antigen. The percentage of T cells and the type of cytokines that are produced give information whether a T cell responds in a suppressive or stimulatory manner. We can distinguish between Th1, Th2, Th17, Tfh and T regulatory cytokines responses. Especially when combined with antigen specificity (CD40L assay and CD8: MHC multimer analyses (combinatorial coding)), this type of assay can help unravel the polarization, strength and specificity of immune responses to immune modulating therapies.

We have a broad range of cytokines that can be measured simultaneously, thereby reducing the amount of cells needed to perform these types of assays.

PBMCsPBMCs from a healthy donor were stimulated with PMA/Ionomycine for 4 hours. The cells were gated on CD3+ and CD4+ cells, showing IL-2 and IL-4 production.

Also see:

References

De Wit J, Jorritsma T, Makuch M, Remmerswaal EB, Klaasse Bos H, Souwer Y, Neefjes J, ten Berge IJ, van Ham SM. Human B cells promote T-cell plasticity to optimize antibody response by inducing coexpression of T(H)1/T(FH) signatures. J Allergy Clin Immunol 2015; 135(4):1053-60.

Boks MA, Kager-Groenland JR, Mousset CM, van Ham SM, ten Brinke A. Inhibition of TNF receptor signalling by anti-TNF╬▒ biologicals primes naïve CD4+ T cells towards IL-10+ T cells with a regulatory phenotype and function. Clin Immunol 2014; 151(2):136-45.

De Wit J, Souwer Y, van Beelen AJ, de Groot R, Muller FJ, Klaasse Bos H, Jorritsma T, Kapsenberg ML, de Jong EC, van Ham SM. CD5 costimulation induces stable Th17 development by promoting IL-23R expression and sustained STAT3 activation. Blood 2011; 118(23):6107-14.

Ten Brinke A, van Schijndel G, Visser R, de Gruijl TD, Zwaginga JJ, van Ham SM. Monophosphoryl lipid A plus IFNgamma maturation of dendritic cells induces antigen-specific CD8(+) cytotoxic T cells with high cytolytic potential. Cancer Immunol Immunother 2010; 59(8):1185-95

Last edited on: 4 November 2015