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Neeltje (NA) Kootstra PhD

Department: Clinical Viro-Immunology

E-mail: n.kootstra@sanquin.nl

Training: Virology and Molecular Biology

Thesis:
10 September 1999
"HIV-1 Replication in Macrophages"
Promotor: prof. F. Miedema (Sanquin Research)
Copromotor: dr H. Schuitemaker (Sanquin Research)
University of Amsterdam

Research interests:
The HIV-1 based lentiviral vector has the ability to integrate its proviral DNA into the genome of dividing and non-dividing cells. Therefore, this vector has great potential for gene therapy, especially for the treatment of hereditary diseases that require life long expression of the transgene. Recently it became clear that gene delivery using the HIV-1 lentiviral vector is restricted in certain tissues and species, and this might limit the use of this vector for gene therapy. To design a HIV-1 based lentiviral that is able to transduce a wide range of target cells/tissue of different species, we study the interaction between viral and cellular proteins and their role during early steps in HIV-1 infection.

Technology: HIV-1 isolation and culture; molecular cloning; confocal microscopy; FACS analysis; analysis of viral fitness; analysis of viral evolution by sequencing; analysis of HIV-1 replication cycle using real time PCR.

Resume:

1999:
PhD. Faculty of Medicine, University of Amsterdam

2000-2002:
Research Associate, Lab of Genetics (group Prof. I.M. Verma), Salk Institute, La Jolla USA

2003-present:
Senior Scientist, Dept of Clinical Viro Immunology (head Prof. H. Schuitemaker), Sanquin Research, Amsterdam

Sanquin publications

Other publications
  • Kootstra, NA, Matsumura R and Verma I.M. Efficient production of human FVIII in hemophilic mice using lentiviral vectors. Mol Ther 2003; 7:623-31.

  • Kootstra, NA, Munk C, Tonnu N,. Landau NR and Verma IM. Abrogation of postentry restriction of HIV-1-based lentiviral vector transduction in simian cells. Proc Natl Acad Sci U S A 2003; 100:1298-303.

  • Kootstra, NA and Verma IM. Gene therapy with viral vectors. Annu Rev Pharmacol Toxicol 2003; 43:413-39.

  • MacKenzie, T, Kobinger G, Kootstra N, Radu A, Sena-Esteves M, Bouchard S, Wilson J, Verma I and Flake A. Efficient transduction of liver and muscle after in utero injection of lentiviral vectors with different pseudotypes. Mol Ther 2002; 6:349.

  • Pfeifer, A, Brandon EP, Kootstra N, Gage FH and Verma IM. Delivery of the Cre recombinase by a self- deleting lentiviral vector: Efficient gene targeting in vivo. Proc Natl Acad Sci U S A 2001; 98:11450-5.

  • Pfeifer, A, Kessler T, Yang M, Baranov E, Kootstra N, Cheresh DA, Hoffman RM and. Verma IM. Transduction of liver cells by lentiviral vectors: analysis in living animals by fluorescence imaging. Mol Ther 2001; 3:319-22.

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